Coding
NarL

Part:BBa_M33030:Design

Designed by: Scott Runyon   Group: Stanford BIOE44 - S010   (2010-06-02)


NarL: Regulates nitrate reduction (strong RBS)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 230
    Illegal BglII site found at 398
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Initial NarL part was ordered with BBa sites but without promoter or RBS. Primers were ordered separately that added these and of course a new set of identical restriction sites, the original ones removed.


Source

Coding sequence taken from NCBI: http://www.ncbi.nlm.nih.gov/gene/945795 and synthesized in Palo Alto by DNA 2.0


References